The procedure is said to have caused Rivers' vocal chords to seize and cut off her air supply.
The Daily News source called it a "huge no-no" and said, "If she had been in a hospital when it happened, she might have been okay."
Rivers died a week after the incident and a state medical examiner completed an autopsy but was not able to determine the cause of death.
In 1985, Rivers appeared on Good Morning America, where she was asked about her heart arrhythmia, a condition in which the heart unexpectedly beats out of sync...something Rivers says scared the hell out of her.
"All of a sudden you'll be on stage and hear 'tha-thud!...It scares the hell out of me."
The State Health Department is investigating the clinic, but currently it is not a criminal investigation.
A source told New York Daily News an unplanned throat biopsy cut off her air supply and possibly killed Joan Rivers.
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